HPLC ANALYSIS OF ATRAZINE IN LOCAL WATER AND IN FISH


Team Members

Kenya Madric
Charles Taft

(Mentor: Mamudu Yakubu)



ABSTRACT

Pesticides are compounds that used to control rodents, insects, weeds, fungi, and anything that is considered to be a "pest". They are used in agriculture to control weeds in crops. For instance, Atrazine(I) (6-chloro-N-ethyl-N'-(1-methylethyl)-1,3,5- triazine-2,4-diamine or 2-chloro-4-ethylamino-6-isopropyl amino-s-triazine) is used to kill weeds in corn fields and sugar cane.

High-performance liquid chromotagraphy (HPLC) has been used to study local water from Pamlico Sound and Weeksville Road creek. The water samples were extracted using chloroform. The organic layer was dried and the solvent was removed by rotary evaporation. The extracts were dissolved in methanol and analyzed using HPLC. A comparison of the retention times obtained for the extracts and standard atrazine shows that atrazine is present in the water samples. The concentration of atrazine in the sample from Pamlico Sound was found to be 0.1433 mg atrazine/mL and that from Weeksville Road creek was 0.01144 mg atrazine/mL.