RONALD E. McNAIR POST BACCALAUREATE ACHIEVEMENT PROGRAM
Director of the Program: Dr. Cheryl J. Lewis
Mentor: Dr. Michael Winrow
Group Members: Tracsa Williams Rodshawn Branch, and Denita Dalton
ABSTRACT
Properties of DNA Fragments that are Associated with Valine Resistance
to Acetohydroxy Acid Synthase Activity in Escherichia coli K-12
Escherichia coli (E. coli) strain K-12 in minimal salt medium is inhibited
by L-valine. This growth sensitivity is largely attributed to the sensitivity of
acetohydroxy acid synthase (AHAS) to feedback inhibition by valine. A cloned DNA fragment
from E. coli that possesses valine resistant AHAS activity thus can support growth of
valine-supplemented minimal medium. In this study, the cloned fragment was
transformed into different strains of E. coli and monitored to see if these
transformants acquired the abiltiy to grow on valine minimal media plates.
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